Carbohydrate Metabolism in Rhizobium trifolii: Identification and Symbiotic Properties of Mutants
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چکیده
منابع مشابه
Sugar Metabolism and the Symbiotic Properties of Carbohydrate Mutants of Rhizobium leguminosarum
Rhizobium leguminosarum metabolizes sugars via the Entner-Doudoroff and pentose phosphate pathways but does not have a functional Embden-Meyerhof pathway. Although some sugar catabolizing enzymes are constitutive, activities of the ‘Entner-Doudoroff enzymes vary with the carbon source. Bacteroids have complete pathways for sugar catabolism even though the specific activities of some enzymes, e....
متن کاملRespiratory Enzyme Systems in Symbiotic Nitrogen Fixation: III. The Dehydrogenase Systems of Rhizobium trifolii and Rhizobium leguminosarum.
Earlier studies on the respiratory systems in symbiotic nitrogen fixation (Wilson, 1938) indicated that the root nodule bacteria (Rhizobium spp.) reduce methylene blue anerobically in the presence of many organic compounds. With few exceptions, the efficiency of a given substrate as a hydrogen donator was approximately the same whether molecular oxygen or methylene blue was used as the hydrogen...
متن کاملTransposon mediation allows a symbiotic plasmid of Rhizobium leguminosarum bv. trifolii to become a symbiosis island in Agrobacterium and Rhizobium.
The symbiotic plasmid (pSym) of Rhizobium leguminosarum bv. trifolii 4S5, which carries Tn5-mob, was successfully transferred into Agrobacterium tumefaciens A136 by using a conjugation method. The resulting transconjugants induced the development of ineffective nitrogen-fixing nodules on the roots of white clover seedlings. Depending on the manner in which the pSym was retained, the transconjug...
متن کاملMolecular mechanism for loss of nodulation properties of Rhizobium trifolii.
Of 18 Rhizobium trifolii strains tested, 12 showed a high frequency of loss of nodulation ability after incubation in cultures at elevated temperatures. A correlation between loss of nodulation ability and loss of a large plasmid was demonstrated for R. trifolii. In some nonnodulating (Nod-) mutants, deletions occurred instead of total elimination of the plasmid molecule. The maximum curing eff...
متن کاملSymbiotic properties of C4-dicarboxylic acid transport mutants of Rhizobium leguminosarum.
The transport of succinate was studied in bacteroids of an effective, streptomycin-resistant strain (GF160) of Rhizobium leguminosarum. High levels of succinate transport occurred, and the kinetics, specificity, and sensitivity to metabolic inhibitors were similar to those previously described for free-living cells. The symbiotic properties of two transposon (Tn5)-mediated C4-dicarboxylate tran...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1979
ISSN: 0022-1287
DOI: 10.1099/00221287-112-1-77